Poly-gamma-glutamic acid differentially alters the abundances and communities of N functional genes involved in urea hydrolysis, nitrification and denitrification when applied with different nitrogen fertilizers

doi:10.1016/j.apsoil.2023.105015

	Poly-gamma-glutamic acid differentially alters the abundances and communities of N functional genes involved in urea hydrolysis, nitrification and denitrification when applied with different nitrogen fertilizers
	Zhang, Lei 1; Gao, Jichao 2; Wang, Lingli 1; Sun, Yu 3; Dong, Xiaoyan 4; Pei, Jiubo 5; Wei, Zhanbo 1; Zhang, Lili 1; Shi, Yuanliang 1
发表期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
	2023-10-01
卷号	190 页码:16
关键词	Poly-gamma-glutamic acid (gamma-PGA) Urea Ammonium sulphate N functional genes Community structure
DOI	10.1016/j.apsoil.2023.105015
通讯作者	Zhang, Lili([email protected]) ; Shi, Yuanliang([email protected])
英文摘要	Poly-gamma-glutamic acid (gamma-PGA), a fertilizer synergist, has a significant promotional effect on nitrogen (N) fertilizer efficiency and crop production by regulating N transformation in soil. However, the underlying mechanism of its effect on soil N cycling is not well understood. The effects of gamma-PGA on the soil properties, extracellular enzyme activities (EEA), abundances and community structures of N functional genes (NFGs) involved in urea (UF) hydrolysis (ureC), nitrification (ammonia oxidation archaea amoA (Arch-amoA) and bacterial amoA (Bacterial-amoA)), denitrification (narG, nirK, qnorB, nosZ) and dissimilatory N reduction (nrfA) were investigated in this study by applying gamma-PGA with different N fertilizers (UF; ammonium sulfate, ASF), so as to reveal the microbial mechanism of gamma-PGA on soil N transformation. The results showed that pH, electrical conductivity, NH4+-N and NO3-N content in soil were greatly and differently affected by gamma-PGA when applied with UF and ASF. gamma-PGA significantly stimulated the microbial biomass N content and EEA activities, except that gamma-PGA significantly inhibited the activities of urease and beta-1,4-glucosidase when applied with UF. gamma-PGA, with UF fertilization, significantly reduced the abundance of ureC, Arch-amoA, Bacterial-amoA and nrfA and significantly increased that of qnorB and nosZ, while gamma-PGA, with ASF fertilization, significantly improved the abundance of Arch-amoA, Bacterial-amoA, qnorB and nosZ and significantly reduced that of narG and nirK. Soil pH and beta-1,4-N-acetylglucosaminidase activity were the best explanatory variables for the abundance variations of NFGs. gamma-PGA greatly affected the community diversity and richness of Arch-amoA, Bacterial-amoA, narG, qnorB and nosZ, and evidently changed the community composition of all NFGs and showed different effects when applied with different N fertilizers. Our results demonstrate that gamma-PGA regulates soil N transformation by altering the abundance and community of NFGs and showed different alterations when applied with different N fertilizers.
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Liaoning Take the Lead Project of 2021: Arable Land Productivity Improvement Strategies Development and Promotion
收录类别	SCI
语种	英语
关键词[WOS]	AMMONIA-OXIDIZING ARCHAEA ; 16S RIBOSOMAL-RNA ; BACTERIAL DIVERSITY ; MICROBIAL BIOMASS ; SOIL BACTERIAL ; POLY(GAMMA-GLUTAMIC ACID) ; EXTRACTION METHOD ; OXIDE REDUCTASE ; USE EFFICIENCY ; PH
研究领域[WOS]	Agriculture
WOS记录号	WOS:001038250900001
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33421
专题	中国科学院烟台海岸带研究所知识产出
通讯作者	Zhang, Lili; Shi, Yuanliang
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China 2.Jinlin Acad Agr Sci, Inst Agr Resources & Environm, 1363 Shengtai St, Changchun 130033, Jilin, Peoples R China 3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, 4888 Shengbei Rd, Changchun 130102, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China 5.Shenyang Agr Univ, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lei,Gao, Jichao,Wang, Lingli,et al. Poly-gamma-glutamic acid differentially alters the abundances and communities of N functional genes involved in urea hydrolysis, nitrification and denitrification when applied with different nitrogen fertilizers[J]. APPLIED SOIL ECOLOGY,2023,190:16.
APA	Zhang, Lei.,Gao, Jichao.,Wang, Lingli.,Sun, Yu.,Dong, Xiaoyan.,...&Shi, Yuanliang.(2023).Poly-gamma-glutamic acid differentially alters the abundances and communities of N functional genes involved in urea hydrolysis, nitrification and denitrification when applied with different nitrogen fertilizers.APPLIED SOIL ECOLOGY,190,16.
MLA	Zhang, Lei,et al."Poly-gamma-glutamic acid differentially alters the abundances and communities of N functional genes involved in urea hydrolysis, nitrification and denitrification when applied with different nitrogen fertilizers".APPLIED SOIL ECOLOGY 190(2023):16.