Genome-resolved metagenomics provides insights into the microbial-mediated sulfur and nitrogen cycling in temperate seagrass meadows

doi:10.3389/fmars.2023.1245288

	Genome-resolved metagenomics provides insights into the microbial-mediated sulfur and nitrogen cycling in temperate seagrass meadows
	Liu, Pengyuan 1,2; Zou, Songbao 3,4,5; Zhang, Haikun1,6 ; Liu, Qing 1,2; Song, Zenglei 1,2; Huang, Yanyan 1,2; Hu, Xiaoke1,2,6
发表期刊	FRONTIERS IN MARINE SCIENCE
	2023-11-28
卷号	10 页码:14
关键词	metagenomics biogeochemical cycling environmental drivers seagrass sediments plant-microbe interactions
DOI	10.3389/fmars.2023.1245288
通讯作者	Hu, Xiaoke([email protected])
英文摘要	The presence of seagrasses facilitates numerous microbial-mediated biogeochemical cycles, with sulfur- and nitrogen-cycling microorganisms playing crucial roles as regulators. Despite efforts to comprehend the diversity of microbes in seagrass ecosystems, the metabolic functions of these benthic microorganisms in seagrass sediments remain largely unknown. Using metagenomics, we provide insights into the sulfur- and nitrogen-cycling pathways and key metabolic capacities of microorganisms in both Z. japonica-colonized and unvegetated sediments over a seasonal period. Taxonomic analysis of N and S cycling genes revealed that delta- and gamma- proteobacteria dominated the benthic sulfate-reducing bacteria, while alpha- and gamma-proteobacteria played a significant role in the sulfur-oxidation processes. The proteobacterial lineages were also major contributors to the benthic nitrogen cycling. However, at a finer taxonomic resolution, microbial participants in different processes were observed to be highly diverse and mainly driven by environmental factors such as temperature and salinity. The gene pools of sulfur and nitrogen cycles in the seagrass sediments were dominated by genes involved in sulfide oxidation (fccA) and hydroxylamine oxidation (hao), respectively. Seagrass colonization elevated the relative abundance of genes responsible for sulfite production (phsC), hydroxylamine oxidation (hao), and nitrogen fixation (nifK), but suppressed sulfur oxidation (soxXYZ) and denitrification (nosZ and nirS). The prevalence of proteobacterial lineages functioned with versatile capabilities in both sulfur and nitrogen cycles in seagrass ecosystems, highlighting tight couplings between these processes, which was further supported by the recovery of 83 metagenome-assembled genomes (MAGs). These findings broaden our understanding of the biogeochemical processes that are mediated by microorganisms in seagrass ecosystems.
资助机构	National Key Research and Development Program ; National Natural Science Foundation of China ; Open Research Fund of National Key Laboratory of Coastal Ecological Environment ; Jilin Province and Chinese Academy of Sciences Technology Cooperation High-Tech Industrialization Special Project
收录类别	SCI
语种	英语
关键词[WOS]	SULFATE-REDUCING BACTERIA ; EELGRASS ZOSTERA-MARINA ; ENHANCES SULFIDE STRESS ; SUBUNIT RIBOSOMAL-RNA ; RIVER ESTUARY ; COMMUNITIES ; DIVERSITY ; REDUCTION ; FIXATION ; HYDROXYLAMINE
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS记录号	WOS:001122457000001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33172
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Hu, Xiaoke
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Minist Agr & Rural Affairs, Zhejiang Inst Freshwater Fisheries, Key Lab Hlth Freshwater Aquaculture, Huzhou, Zhejiang, Peoples R China 4.Zhejiang Inst Freshwater Fisheries, Key Lab Fish Hlth & Nutr Zhejiang Prov, Huzhou, Zhejiang, Peoples R China 5.Zhejiang Inst Freshwater Fisheries, Huzhou Key Lab Aquat Prod Qual Improvement & Proc, Huzhou, Zhejiang, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Pengyuan,Zou, Songbao,Zhang, Haikun,et al. Genome-resolved metagenomics provides insights into the microbial-mediated sulfur and nitrogen cycling in temperate seagrass meadows[J]. FRONTIERS IN MARINE SCIENCE,2023,10:14.
APA	Liu, Pengyuan.,Zou, Songbao.,Zhang, Haikun.,Liu, Qing.,Song, Zenglei.,...&Hu, Xiaoke.(2023).Genome-resolved metagenomics provides insights into the microbial-mediated sulfur and nitrogen cycling in temperate seagrass meadows.FRONTIERS IN MARINE SCIENCE,10,14.
MLA	Liu, Pengyuan,et al."Genome-resolved metagenomics provides insights into the microbial-mediated sulfur and nitrogen cycling in temperate seagrass meadows".FRONTIERS IN MARINE SCIENCE 10(2023):14.