Screening of alginate lyase-excreting microorganisms from the surface of brown algae

doi:10.1186/s13568-017-0361-x

	Screening of alginate lyase-excreting microorganisms from the surface of brown algae
	Wang,Mingpeng 1; Chen,Lei 2; Zhang,Zhaojie 3; Wang,Xuejiang 4; Qin,Song2 ; Yan,Peisheng 1,5
发表期刊	AMB Express
ISSN	2191-0855
	2017-04-04
卷号	7 期号:1
关键词	Alginate lyase Gram’s iodine Alginate degradation Screening Brown algae
DOI	10.1186/s13568-017-0361-x
通讯作者	Qin,Song([email protected]) ; Yan,Peisheng([email protected])
英文摘要	AbstractAlginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum were cultured in sterile water. Bacteria growing on the surface of seaweeds were identified and their capacity of excreting alginate lyase was analyzed. A total of 196 strains were recovered from the three different algae samples and 12 different bacterial strains were identified capable of excreting alginate lyases. Sequence analysis of the 16S rRNA gene revealed that these alginate lyase-excreting strains belong to eight genera: Paenibacillus (4/12), Bacillus (2/12), Leclercia (1/12), Isoptericola (1/12), Planomicrobium (1/12), Pseudomonas (1/12), Lysinibacillus (1/12) and Sphingomonas (1/12). Further analysis showed that the LJ-3 strain (Bacillus halosaccharovorans) had the highest enzyme activity. To our best knowledge, this is the first report regarding alginate lyase-excreting strains in Paenibacillus, Planomicrobium and Leclercia. We believe that our method used in this study is relatively easy and reliable for large-scale screening of alginate lyase-excreting microorganisms.
语种	英语
WOS记录号	BMC:10.1186/s13568-017-0361-x
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32095
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Qin,Song; Yan,Peisheng
作者单位	1.Harbin Institute of Technology; School of Municipal and Environmental Engineering 2.Yantai Institute of Costal Zone Research Chinese Academy of Sciences 3.University of Wyoming; Department of Zoology and Physiology 4.Worldfull Agricultural Science and Technology Co., Ltd 5.Harbin Institute of Technology at Weihai; School of Marine Science and Technology
推荐引用方式 GB/T 7714	Wang,Mingpeng,Chen,Lei,Zhang,Zhaojie,et al. Screening of alginate lyase-excreting microorganisms from the surface of brown algae[J]. AMB Express,2017,7(1).
APA	Wang,Mingpeng,Chen,Lei,Zhang,Zhaojie,Wang,Xuejiang,Qin,Song,&Yan,Peisheng.(2017).Screening of alginate lyase-excreting microorganisms from the surface of brown algae.AMB Express,7(1).
MLA	Wang,Mingpeng,et al."Screening of alginate lyase-excreting microorganisms from the surface of brown algae".AMB Express 7.1(2017).