Effect of mixed inhibitor application on N2O production pathways in paddy soil

doi:10.1007/s11368-022-03183-2

	Effect of mixed inhibitor application on N2O production pathways in paddy soil
	Wu, Kaikuo 1,2; Gong, Ping 1,3,4; Bai, Wei 5,6; Zhang, Zhe 5,6; Wei, Zhanbo 1,4; Yu, Chunxiao 7; Song, Yuchao 1,3,4; Xue, Yan 1,3,4; Zhang, Lili 1,3,4
发表期刊	JOURNAL OF SOILS AND SEDIMENTS
ISSN	1439-0108
	2022-03-28
页码	11
关键词	Inhibitors Paddy soil N2O production Ammonia oxidation Heterotrophic denitrification
DOI	10.1007/s11368-022-03183-2
通讯作者	Zhang, Lili([email protected])
英文摘要	Purpose In flooded paddy soils, quantifying and discerning nitrous oxide (N2O) production by four biological pathways (nitrifier nitrification (NN), nitrifier denitrification (ND), nitrification-coupled denitrification (NCD), and heterotrophic denitrification (HD)) are essential for developing innovative strategies to mitigate the greenhouse effect. Materials and methods Soils were collected from Shenyang Experimental Station of the Institute of Applied Ecology, Liaoning Province, China, and were sealed and incubated in the dark at 25 degrees C and submerged for 48 h. The amount of N2O produced by each of the four pathways and the abundance of corresponding functional genes were determined by dual-isotope (N-15-O-18) labeling technique combined with quantitative PCR (qPCR). Results and discussion In our incubation experiment, N-15 isotope tracing showed that not urea but paddy soil was the largest contributor of N2O within 48 h after urea application. Combined application of urea and mixed inhibitors (N-(n-butyl) thiophosphoric triamide (NBPT) + phenylphosphorodiamidate (PPD) + 3,4-dimethylpyrazole phosphate (DMPP)) could reduce total N2O production by 26.69%. Through dual-isotope (O-18-N-15) labeling technology, it was found that N2O production mainly came from HD pathway, accounting for 77% of total N2O production, and N2O produced by ammonia oxidation (NN, ND, and NCD) after urea application accounted for 23% of total N2O production. The largest proportion of N2O production among the ammonia oxidation pathways was the ND pathway (0-23.00%), followed by the NN pathway (0-19.21%) and NCD pathway (0-3.79%). Application of mixed inhibitors significantly reduced the N2O produced by the HD pathway by more than 15%; reduced the N2O produced by the ammonia oxidation pathway from 23.00 to 10.39%; and reduced the N2O produced by the NN, ND, and NCD pathways by more than 50%. This is probably caused by the decreased ammonium level and reduced gene copies of AOB amoA, narG, and nirK, which are key N2O producing genes. Conclusions Incubation experiment showed that ammonia oxidation pathway is also an important pathway for N2O production in flooded paddy soil. Mixed inhibitors have inhibitory effects on N2O produced by NN, ND, NCD, and HD pathways. The future development of mixed inhibitor application strategies suitable for paddy fields is of great significance for mitigating the global greenhouse effect.
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Scientific Foundation Project of China ; Major Science and Technology Project of Liaoning Province ; Special scientific and technological innovation project for agricultural green and high-quality development ; Liaoning Take the Lead Project of 2021: Arable Land Productivity improvement strategies development and promotion
收录类别	SCI
语种	英语
关键词[WOS]	NITROUS-OXIDE EMISSIONS ; GREENHOUSE-GAS EMISSIONS ; NITRIFIER DENITRIFICATION ; FERTILIZER N ; AMMONIA LOSS ; NITRIFICATION ; UREA ; MITIGATION ; MANAGEMENT ; EFFICIENCY
研究领域[WOS]	Environmental Sciences & Ecology ; Agriculture
WOS记录号	WOS:000773877700001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31367
专题	中国科学院烟台海岸带研究所知识产出
通讯作者	Zhang, Lili
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Engn Lab Soil Nutrient Management, Shenyang 110016, Peoples R China 4.Chinese Acad Sci, Engn Lab Green Fertilizers, Shenyang 110016, Peoples R China 5.Liaoning Acad Agr Sci, Tillage & Cultivat Res Inst, Liaoning Key Lab Conservat Tillage Dry Land, Shenyang 110016, Peoples R China 6.Natl Agr Expt Stn Agr Environm, Fuxing 123100, Peoples R China 7.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wu, Kaikuo,Gong, Ping,Bai, Wei,et al. Effect of mixed inhibitor application on N2O production pathways in paddy soil[J]. JOURNAL OF SOILS AND SEDIMENTS,2022:11.
APA	Wu, Kaikuo.,Gong, Ping.,Bai, Wei.,Zhang, Zhe.,Wei, Zhanbo.,...&Zhang, Lili.(2022).Effect of mixed inhibitor application on N2O production pathways in paddy soil.JOURNAL OF SOILS AND SEDIMENTS,11.
MLA	Wu, Kaikuo,et al."Effect of mixed inhibitor application on N2O production pathways in paddy soil".JOURNAL OF SOILS AND SEDIMENTS (2022):11.