Mucin corona delays intracellular trafficking and alleviates cytotoxicity of nanoplastic-benzopyrene combined contaminant

doi:10.1016/j.jhazmat.2020.124306

	Mucin corona delays intracellular trafficking and alleviates cytotoxicity of nanoplastic-benzopyrene combined contaminant
	Ji, Yunxia 1,2; Wang, Yunqing2,4 ; Shen, Dazhong 1; Kang, Qi 1; Chen, Lingxin2,3,4,5
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
	2021-03-15
卷号	406 页码:13
关键词	Nanoplastics Combined contamination Protein corona Intracellular trafficking Toxicological effects
DOI	10.1016/j.jhazmat.2020.124306
通讯作者	Kang, Qi([email protected]) ; Chen, Lingxin([email protected])
英文摘要	Nanoplastics have recently become a worldwide concern as newly emerging airborne pollutants, which can associate with polycyclic aromatic hydrocarbons (PAHs) and form combined contaminant nanoparticles (CCNPs). After being inhaled in the respiratory system, the CCNPs would first encounter the mucous gel layer being rich in mucin. Herein, polystyrene-benzopyrene (PS@Bap) NPs were prepared as CCNPs model and their interaction with mucin and the resultant biological responses were studied. It was observed that mucin corona stably attached to the CCNPs surface, which significantly altered the fate of the CCNPs in lung epithelial cells (A 549 cell line). The mucin corona would 1) stably adsorbed on PS@Bap at the early stages of endocytosis until degraded during the lysosomal transport and maturation process, 2) delay intracellular trafficking of PS@Bap and the progress of Bap detached from PS, 3) enhance uptake of PS@Bap but reduce the cytotoxicity elicited by PS@Bap, as indicated by cell viability, generation of reactive oxygen species, impairment on mitochondrial function, and further cell apoptosis. In addition, in vivo study also verified the enhanced effect of PS on the development of an acute lung inflammatory response induced by Bap. This study highlights the significance of incorporating the effects of mucin for precisely assessing the respiratory system toxicity of nanoplastics based CCNPs in atmospheric environments.
资助机构	National Natural Science Foundation of China ; program of Youth Innovation Promotion Association, CAS ; Instrument Developing Project of the Chinese Academy of Sciences ; Taishan Scholar Project Special Fund
收录类别	SCI
语种	英语
关键词[WOS]	POTENTIAL HEALTH IMPACT ; POLYSTYRENE NANOPARTICLES ; MICROPLASTICS ; MICRO ; ROS ; ACTIVATION ; PARTICLES ; APOPTOSIS ; SORPTION ; BARRIER
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:000701704400005
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30760
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Kang, Qi; Chen, Lingxin
作者单位	1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Ji, Yunxia,Wang, Yunqing,Shen, Dazhong,et al. Mucin corona delays intracellular trafficking and alleviates cytotoxicity of nanoplastic-benzopyrene combined contaminant[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,406:13.
APA	Ji, Yunxia,Wang, Yunqing,Shen, Dazhong,Kang, Qi,&Chen, Lingxin.(2021).Mucin corona delays intracellular trafficking and alleviates cytotoxicity of nanoplastic-benzopyrene combined contaminant.JOURNAL OF HAZARDOUS MATERIALS,406,13.
MLA	Ji, Yunxia,et al."Mucin corona delays intracellular trafficking and alleviates cytotoxicity of nanoplastic-benzopyrene combined contaminant".JOURNAL OF HAZARDOUS MATERIALS 406(2021):13.