| Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana | |
Wang, Wenjing; Sheng, Yanqing
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| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 2021-01 | |
| 卷号 | 37期号:1页码:3 |
| 关键词 | Ampicillin Carotenoid biosynthesis Chlorella sorokiniana Fatty acid biosynthesis Porphyrin and chlorophyll metabolism |
| DOI | 10.1007/s11274-020-02985-1 |
| 英文摘要 | Ampicillin sodium salt (AMP) is commonly and effectively used to prevent bacterial infection in algal culture, but the response of algal strains to AMP has not been investigated. In this study, Chlorella sorokiniana was selected to evaluate the influence of AMP on algae. AMP enhanced the contents of chlorophyll and two fatty acids, myristic acid (C22:1N9) and tetracosanoic acid (C6:0), but inhibited the growth, carotenoid production, and contents of 16 fatty acids in C. sorokiniana. A global transcriptome analysis from experimental data identified 3 825 upregulated and 1 432 downregulated differentially expressed genes (DEGs) in C. sorokiniana. The upregulated DEGs, such as hemB/alaD, mmaB/pduO, cox15/ctaA, fxN, cpoX/hemF, and earS/gltX, were enriched in the porphyrin and chlorophyll metabolism pathways, whereas the downregulated DEGs, including lcyB (crtL1), crtY (lcyE, crtL2), lut1 (CYP97C1), z-isO, crtZ and crtisO (crtH), were enriched in the carotenoid biosynthesis pathway, and the downregulated DEGs, abH, fadD, fabF, acsL, fabG, and accD were enriched in the fatty acid biosynthesis pathway. Thus, the use of AMP to obtain an axenic strain revealed that AMP might affect the regulatory dynamics and the results of the metabolic process in C. sorokiniana. The data obtained in the study provide foundational information for algal purification and aseptic processing.Graphic abstract |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31600370, 41373100] ; Science and Technology Plan of Yantai City [2018ZHGY080, 2018ZHGY083] ; Regional Key Project of STS of the Chinese Academy of Sciences [KFJ-STS-QYZX-057] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RNA-SEQ DATA ; AXENIC CULTURES ; GENE-TRANSFER ; BACTERIA ; CAROTENOIDS ; ALGAE ; ANTIBIOTICS ; CULTIVATION ; EXPRESSION ; EVOLUTION |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000606495400002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/30506 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 作者单位 | Chinese Acad Sci, Res Ctr Coastal Environm Engn Technol Shandong Pr, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenjing,Sheng, Yanqing. Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2021,37(1):3. |
| APA | Wang, Wenjing,&Sheng, Yanqing.(2021).Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,37(1),3. |
| MLA | Wang, Wenjing,et al."Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 37.1(2021):3. |
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