| Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress | |
Zhang, Xia1,5; Zhang, Liangwei1,3 ; Wang, Xiaoyan1,4; Han, Xiaoyue1,5; Huang, Yan1,4; Li, Bowei1,3; Chen, Lingxin1,2,3
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| 发表期刊 | JOURNAL OF HAZARDOUS MATERIALS
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| ISSN | 0304-3894 |
| 2021-10-05 | |
| 卷号 | 419页码:10 |
| 关键词 | Fluorescent probe Cysteine Near-infrared imaging Hypoxia reperfusion Environmental stress |
| DOI | 10.1016/j.jhazmat.2021.126476 |
| 通讯作者 | Chen, Lingxin([email protected]) |
| 英文摘要 | Increasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it's obscure how environmental stress disturbs the normal physiological process, and then how endogenous reactive species mend the cases. Hypoxia/reperfusion (H/R), a common and intractable injury in aquaculture and clinic, can induce oxidative stress and ultimately cause irreversible injury to organism. Cysteine (Cys) plays essential roles in maintaining transduction of numerous reactive species and redox homeostasis in subcellular structures, cells and organisms. A great deal of fluorescence research about Cys are focusing on development of selective probes but with poor exploration of the biofunction under environmental stress. Therefore, it is of great significance to examine the bio-effects of Cys against H/R stress. In the present work, we design a fluorescent probe BCy-AC for in situ detecting Cys, the unique Enol-Keto tautomerization of fluorophore BCy-Keto propels the reaction process which will improve the sensitivity and potential application performance of the probe. BCy-AC is conveniently applied to visualize Cys in HT-22 cells, zebrafish and mice tissues. Moreover, imaging results obtained from H/R models reveal that endogenous Cys changes with hypoxia and reperfusion time and Cys pretreatment effectively defend H/R injury in cells and in vivo. |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Shandong Provincial Natural Science Founda-tion Key Project ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SELECTIVE DETECTION ; GLUTATHIONE DEPLETION ; GOLD NANOPARTICLES ; REACTIVE OXYGEN ; RECENT PROGRESS ; PROBE ; POLYSULFIDES ; LUMINESCENT |
| 研究领域[WOS] | Engineering ; Environmental Sciences & Ecology |
| WOS记录号 | WOS:000694784800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29744 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, Lingxin |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xia,Zhang, Liangwei,Wang, Xiaoyan,et al. Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,419:10. |
| APA | Zhang, Xia.,Zhang, Liangwei.,Wang, Xiaoyan.,Han, Xiaoyue.,Huang, Yan.,...&Chen, Lingxin.(2021).Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress.JOURNAL OF HAZARDOUS MATERIALS,419,10. |
| MLA | Zhang, Xia,et al."Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress".JOURNAL OF HAZARDOUS MATERIALS 419(2021):10. |
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