| Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy | |
Lu, Pengpeng1,2; Zhang, Caiyun1; Fu, Lili1,2; Wei, Yinghui2,3; Huang, Yan1,2; Wang, Xiaoyan1,2; Lv, Changjun3; Chen, Lingxin1,2,4
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 2021-07-27 | |
| 卷号 | 93期号:29页码:10378-10387 |
| DOI | 10.1021/acs.analchem.1c02386 |
| 通讯作者 | Huang, Yan([email protected]) ; Wang, Xiaoyan([email protected]) ; Chen, Lingxin([email protected]) |
| 英文摘要 | Pantetheinase (also known as Vanin-1) is highly expressed in the liver, kidneys, and intestine and is closely associated with a number of diseases. Vanin-1 can hydrolyze pantetheine to pantothenic acid (vitamin B5) and cysteamine and participate in the synthesis of glutathione (GSH). GSH is highly expressed in tumor cells and plays a major role in the resistance of tumor cells to cisplatin. Therefore, we urgently need a method to monitor the activity level of Vanin-1 in tumor cells and tissues and elucidate the relationship between the role of Vanin-1 in GSH synthesis and tumor resistance. Herein, we report a Cy-Pa fluorescent probe for imaging Vanin-1 in cells and in vivo that can qualitatively and quantitatively detect the fluctuation of Vanin-1 concentrations in HepG2 and HepG2/DDP cells or tumor tissues of tumor-bearing mice. This probe shows excellent potential in in situ real-time monitoring of endogenous Vanin-1. Moreover, we proved that Vanin-1 can inhibit GSH synthesis using the probe. When the Vanin-1 inhibitor RR6 was used in combination with cisplatin, HepG2 and HepG2/DDP cells showed increased resistance to cisplatin, while the therapeutic efficiency of cisplatin was reduced in HepG2 and HepG2/DDP xenografts. In this study, Vanin-1 was shown to play an important role in the treatment of cancer, and the study of Vanin-1 may provide an idea for the treatment of cancer in the future. |
| 资助机构 | National Nature Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Fund ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | DRUG-RESISTANCE ; URINARY VANIN-1 ; CANCER ; GLUTATHIONE ; CISPLATIN ; PANTETHEINASE ; ASSAY ; CYSTEAMINE ; METABOLISM ; PATHWAY |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000679366000052 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29552 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Huang, Yan; Wang, Xiaoyan; Chen, Lingxin |
| 作者单位 | 1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Pengpeng,Zhang, Caiyun,Fu, Lili,et al. Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy[J]. ANALYTICAL CHEMISTRY,2021,93(29):10378-10387. |
| APA | Lu, Pengpeng.,Zhang, Caiyun.,Fu, Lili.,Wei, Yinghui.,Huang, Yan.,...&Chen, Lingxin.(2021).Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy.ANALYTICAL CHEMISTRY,93(29),10378-10387. |
| MLA | Lu, Pengpeng,et al."Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy".ANALYTICAL CHEMISTRY 93.29(2021):10378-10387. |
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