| Determination of chitosan content with Schiff base method and HPLC | |
Miao, Qin1,2,3; Mi, Yingqi1,2,3; Cui, Jingmin1,2,3; Zhang, Jingjing1,2; Tan, Wenqiang1,2 ; Li, Qing1,2; Guo, Zhanyong1,2,3
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| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 2021-07-01 | |
| 卷号 | 182页码:1537-1542 |
| 关键词 | Determination of chitosan content Chitosan-Schiff base HPLC |
| DOI | 10.1016/j.ijbiomac.2021.05.121 |
| 通讯作者 | Guo, Zhanyong([email protected]) |
| 英文摘要 | Tremendous awareness of determination of chitosan content accurately is increasing, due to it has great significance to the quality control of chitosan. In this article, two kinds of chitosan-Schiff base derivatives (BCSB and PCSB) were synthesized by the different average degrees of deacetylation (DD) of chitosan with benzaldehyde or propanal, respectively. The total mass of Schiff base derivative product was dried and obtained without washing and loss. Then, a certain amount of the prepared Schiff base compound was taken to hydrolyze into glucosamine hydrochloride (GAH) in strong hydrochloric acidic environment, whose concentration was quantified by HPLC, and the mass of GAH contained in hydrolysis solution could be calculated. Subsequently, the total quality of GAH obtained by hydrolysis of all of the Schiff base product was calculated and obtained, and then the theoretical mass of chitosan could be deduced and calculated by further converse calculation. Finally, the chitosan content was obtained by combining the sample mass used in Schiff base reaction and the theoretical mass of chitosan. This method was accurate and convenient, providing a preeminent idea and method for the determination of chitosan content. (c) 2021 Elsevier B.V. All rights reserved. |
| 资助机构 | National Key Research and Development Program of China ; Natural Science Foundation of Shandong Province of China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LIQUID-CHROMATOGRAPHIC DETERMINATION ; ACID-HYDROLYSIS ; ANTIMICROBIAL ACTIVITY ; GLUCOSAMINE ; CHITIN ; DEACETYLATION ; STABILITY ; SAMPLES ; CRAB |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS记录号 | WOS:000669494700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29531 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Guo, Zhanyong |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, Qin,Mi, Yingqi,Cui, Jingmin,et al. Determination of chitosan content with Schiff base method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,182:1537-1542. |
| APA | Miao, Qin.,Mi, Yingqi.,Cui, Jingmin.,Zhang, Jingjing.,Tan, Wenqiang.,...&Guo, Zhanyong.(2021).Determination of chitosan content with Schiff base method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,182,1537-1542. |
| MLA | Miao, Qin,et al."Determination of chitosan content with Schiff base method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 182(2021):1537-1542. |
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