| Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins | |
Yang, Guilan1,2; Qin, Song1,3 ; Li, Wenjun1,3
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| 发表期刊 | JOURNAL OF FOOD SCIENCE
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| ISSN | 0022-1147 |
| 2021-05-30 | |
| 页码 | 11 |
| 关键词 | Alaska pollack skins protein hydrolysate angiotensin I-converting enzyme (ACE)-inhibitory peptide gel filtration chromatography molecular docking ultrafiltration |
| DOI | 10.1111/1750-3841.15754 |
| 通讯作者 | Li, Wenjun([email protected]) |
| 英文摘要 | Hydrolysates containing angiotensin I-converting enzyme (ACE)-inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G-25 gel filtration chromatography and reversed phase high-performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and beta-sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC-Q-TOF-MS (HPLC-quadrupole time-of-flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31 Da showed the highest ACE inhibitory activity (IC50 = 105.8 mu M). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver-Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE. |
| 资助机构 | National Key Research and Development Program of China ; Marine Economy Innovation Development Demonstration Project |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PROTEIN HYDROLYSATE ; ACE ; STABILITY ; KINETICS ; MUSCLE ; VENOM |
| 研究领域[WOS] | Food Science & Technology |
| WOS记录号 | WOS:000656102700001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29298 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Li, Wenjun |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Guilan,Qin, Song,Li, Wenjun. Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins[J]. JOURNAL OF FOOD SCIENCE,2021:11. |
| APA | Yang, Guilan,Qin, Song,&Li, Wenjun.(2021).Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins.JOURNAL OF FOOD SCIENCE,11. |
| MLA | Yang, Guilan,et al."Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins".JOURNAL OF FOOD SCIENCE (2021):11. |
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| Purification and cha（1714KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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