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| Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides | |
Wang, Qiaoning1,2,5; Jiang, Yaru1; Wang, Hongdan1,2; Chang, Xianbo4; Lv, Min1,2 ; Chen, Lingxin1,2,3
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| 发表期刊 | ENVIRONMENTAL RESEARCH
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| ISSN | 0013-9351 |
| 2020-09-01 | |
| 卷号 | 188页码:12 |
| 关键词 | Sulfonamides Vibrio diabolicus strain L2-2 Acetylation Biotransformation Arylamine N-Acetyltransferase |
| DOI | 10.1016/j.envres.2020.109718 |
| 通讯作者 | Lv, Min([email protected]) ; Chen, Lingxin([email protected]) |
| 英文摘要 | Sulfonamides (SAs) have attracted much attention because of their high detection rates in natural water. In this study, a marine bacterium Vibrio diabolicus strain L2-2 was isolated which could metabolize 9 SAs to a different extent. Compared with SAs and their analogs, SAs with N-oxides of heterocyclic structure were easier to be transformed to their N-4-acetylated metabolites or their isoxazole ring rearrangement isomers by strain L2-2. And, gene vdnatA and vdnatG were likely to be the key genes in SAs acetylation process, which might code Arylamine N-acetyltransferase. The biotransformation rates of sulfathiazole(STZ), sulfamonomethoxine(SMT), sulfadiazine (SDZ), sulfamethoxazole(SMX) and sulfisoxazole(SIX) could reach 29.39 +/- 5.63, 24.97 +/- 4.45, 79.41 +/- 4.05, 64.64 +/- 1.71, 32.82 +/- 4.46% in 6 days, respectively. Besides, the overall optimal conditions for SAs biotransformation were less than 100 mg/L for total SAs in neutral or weakly alkaline medium with the salinity of 10-20%o and additional nutrients like glucose, sucrose or glycerine. Furthermore, toxicity was demonstrated to be significantly reduced after biotransformation. Together, this study introduced a strategy to use V. diabolicus strain L2-2 to realize simultaneous removal and detoxification of multiple SAs in freshwater and seawater, and revealed SAs removal pathways and relevant molecular mechanism. |
| 资助机构 | National Natural Science Foundation of China ; Key Research and Development Project of Shandong Province of China ; Key Research and Development Project of Yantai City ; Science and Technology Development Plan of Yantai ; Taishan Scholar Project Special Funding ; Project of Science and Technology Development of Shandong Province of China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | AMMONIA OXIDIZING BACTERIA ; ACTIVATED-SLUDGE ; SULFAMETHOXAZOLE BIODEGRADATION ; ANTIBIOTIC SULFAMETHOXAZOLE ; TRANSFORMATION PRODUCTS ; VETERINARY ANTIBIOTICS ; MICROBIAL GENOMES ; METABOLIC PATHWAY ; N-ACETYLATION ; SULFA DRUGS |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| WOS记录号 | WOS:000564656800008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/28509 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Lv, Min; Chen, Lingxin |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Yantai Univ, Coll Environm & Mat Engn, Yantai 264005, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qiaoning,Jiang, Yaru,Wang, Hongdan,et al. Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides[J]. ENVIRONMENTAL RESEARCH,2020,188:12. |
| APA | Wang, Qiaoning,Jiang, Yaru,Wang, Hongdan,Chang, Xianbo,Lv, Min,&Chen, Lingxin.(2020).Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides.ENVIRONMENTAL RESEARCH,188,12. |
| MLA | Wang, Qiaoning,et al."Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides".ENVIRONMENTAL RESEARCH 188(2020):12. |
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