| Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571 | |
Liu, Xiaolin1,2; Liu, Yanan1,2; Johnson, Kevin Scot3; Dong, Xiaoyan1,4; Xie, Zhihong1,4,5
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| 发表期刊 | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 2020-12-07 | |
| 卷号 | 11页码:12 |
| 关键词 | chemotaxis CheZ cellular localization Azorhizobium caulinodans rhizobia |
| DOI | 10.3389/fmicb.2020.585140 |
| 通讯作者 | Xie, Zhihong([email protected]) |
| 英文摘要 | Chemotaxis is essential for the competitiveness of motile bacteria in complex and harsh environments. The localization of chemotactic proteins in the cell is critical for coordinating a maximal response to chemotactic signals. One chemotaxis protein with a well-defined subcellular localization is the phosphatase CheZ. CheZ localizes to cell poles by binding with CheA in Escherichia coli and other enteric bacteria, or binding with a poorly understood protein called ChePep in epsilon-Proteobacteria. In alpha-Proteobacteria, CheZ lacks CheA-binding sites, and its cellular localization remains unknown. We therefore determined the localization of CheZ in the alpha-Proteobacteria Azorhizobium caulinodans ORS571. A. caulinodans CheZ, also termed as CheZ(AC,) was found to be located to cell poles independently of CheA, and we suspect that either the N-terminal helix or the four-helix bundle of CheZ(AC) is sufficient to locate to cell poles. We also found a novel motif, AXXFQ, which is adjacent to the phosphatase active motif DXXXQ, which effects the monopolar localization of CheZ(AC). This novel motif consisting of AXXFQ is conserved in CheZ and widely distributed among Proteobacteria. Finally, we found that the substitution of phosphatase active site affects the polar localization of CheZ(AC). In total, this work characterized the localization pattern of CheZ containing a novel motif, and we mapped the regions of CheZ(AC) that are critical for its polar localization. |
| 资助机构 | NSFC-Shandong Joint Fund Key Projects ; National Natural Science Foundation of China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CHEMOTAXIS PHOSPHATASE CHEZ ; ESCHERICHIA-COLI ; POLAR LOCALIZATION ; PHOSPHORYLATED CHEY ; SIGNALING PROTEINS ; COMPLEX ; CHEMORECEPTOR ; BACTERIA ; BINDING ; DIVERSITY |
| 研究领域[WOS] | Microbiology |
| WOS记录号 | WOS:000600397400001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27559 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Xie, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 3.Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,et al. Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12. |
| APA | Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,Dong, Xiaoyan,&Xie, Zhihong.(2020).Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571.FRONTIERS IN MICROBIOLOGY,11,12. |
| MLA | Liu, Xiaolin,et al."Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571".FRONTIERS IN MICROBIOLOGY 11(2020):12. |
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| Protein Residues and（4189KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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