| Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli | |
Li, Wenjun1,2 ; Ma, Chengbo1,3; Ge, Baosheng4; Zang, Fan1,3; Qin, Song1,2; Lin, Jian3
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| 发表期刊 | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| 2021-04-29 | |
| 页码 | 11 |
| 关键词 | Combinational biosynthesis Phycoerythrobilin Response surface methodology Extraction |
| DOI | 10.1007/s10811-021-02408-0 |
| 通讯作者 | Qin, Song([email protected]) ; Lin, Jian([email protected]) |
| 英文摘要 | Phycoerythrobilin (PEB) is a special pigment in red algae and cyanobacteria that plays an important role in capturing light energy. As a natural non-toxic pigment with antioxidant properties, PEB is widely used in food and cosmetics industries. At present, the presence of other pigments in red algae and cyanobacteria, such as chlorophyll and phycocyanobilin, makes the preparation of PEB complicated and costly. In this study, the PEB synthesizing enzyme genes of hox1 from Synechocystis sp. PCC6803 and pebS from Nostoc sp. PCC 7120 were co-expressed in Escherichia coli to produce recombinant PEB. In addition, single-factor and response surface methods were used to enhance the yield of recombinant PEB. Under the conditions of lactose concentration at 4 mmol L-1, induction temperature at 27 degrees C, OD600 nm of 0.9, and induction time at 14 h, the concentration of PEB reached 20.37 mg L-1. In addition, a high-purity (purity > 70%) PEB was obtained through chloroform extraction, and the purified product was verified in UV spectrometry, high-performance liquid chromatography, and mass spectrometry. |
| 资助机构 | National Natural Science Foundation of China ; Key Research and Development Program of Yantai City, Shandong, China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000645498300002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27224 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Qin, Song; Lin, Jian |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenjun,Ma, Chengbo,Ge, Baosheng,et al. Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:11. |
| APA | Li, Wenjun,Ma, Chengbo,Ge, Baosheng,Zang, Fan,Qin, Song,&Lin, Jian.(2021).Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli.JOURNAL OF APPLIED PHYCOLOGY,11. |
| MLA | Li, Wenjun,et al."Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli".JOURNAL OF APPLIED PHYCOLOGY (2021):11. |
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| Biosynthesis and pre（1586KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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