| Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant | |
Sun, Yu; Liu, Yanan; Liu, Xiaolin; Dang, Xiaoxiao; Dong, Xiaoyan; Xie, Zhihong
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| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| ISSN | 0175-7598 |
| 2020-03 | |
| 卷号 | 104期号:6页码:2715-2729 |
| 关键词 | CYCLIC DIGUANYLATE PSEUDOMONAS-AERUGINOSA SINORHIZOBIUM-MELILOTI SESBANIA-ROSTRATA ACETOBACTER-XYLINUM ALLOSTERIC CONTROL CRYSTAL-STRUCTURES BIOFILM FORMATION VIBRIO-CHOLERAE PAS DOMAINS |
| 研究领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s00253-020-10404-6 |
| 产权排序 | [Sun, Yu; Liu, Yanan; Liu, Xiaolin; Dang, Xiaoxiao; Dong, Xiaoyan; Xie, Zhihong] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Sun, Yu] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Changchun, Peoples R China; [Liu, Yanan; Liu, Xiaolin; Dang, Xiaoxiao] Univ Chinese Acad Sci, Beijing, Peoples R China; [Dong, Xiaoyan; Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Peoples R China |
| 通讯作者 | Xie, Zhihong([email protected]) |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Establishment of the rhizobia-legume symbiosis is usually accompanied by hydrogen peroxide (H2O2) production by the legume host at the site of infection, a process detrimental to rhizobia. In Azorhizobium caulinodans ORS571, deletion of chp1, a gene encoding c-di-GMP phosphodiesterase, led to increased resistance against H2O2 and to elevated nodulation efficiency on its legume host Sesbania rostrata. Three domains were identified in the Chp1: a PAS domain, a degenerate GGDEF domain, and an EAL domain. An in vitro enzymatic activity assay showed that the degenerate GGDEF domain of Chp1 did not have diguanylate cyclase activity. The phosphodiesterase activity of Chp1 was attributed to its EAL domain which could hydrolyse c-di-GMP into pGpG. The PAS domain functioned as a regulatory domain by sensing oxygen. Deletion of Chp1 resulted in increased intracellular c-di-GMP level, decreased motility, increased aggregation, and increased EPS (extracellular polysaccharide) production. H2O2-sensitivity assay showed that increased EPS production could provide ORS571 with resistance against H2O2. Thus, the elevated nodulation efficiency of the increment chp1 mutant could be correlated with a protective role of EPS in the nodulation process. These data suggest that c-di-GMP may modulate the A. caulinodans-S. rostrata nodulation process by regulating the production of EPS which could protect rhizobia against H2O2. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050102] ; NSFC-Shandong Joint Fund Key Projects [U1806206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31870020, 31570063] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CYCLIC DIGUANYLATE ; PSEUDOMONAS-AERUGINOSA ; SINORHIZOBIUM-MELILOTI ; SESBANIA-ROSTRATA ; ACETOBACTER-XYLINUM ; ALLOSTERIC CONTROL ; CRYSTAL-STRUCTURES ; BIOFILM FORMATION ; VIBRIO-CHOLERAE ; PAS DOMAINS |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000510299300002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/25095 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Changchun, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yu,Liu, Yanan,Liu, Xiaolin,et al. Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(6):2715-2729. |
| APA | Sun, Yu,Liu, Yanan,Liu, Xiaolin,Dang, Xiaoxiao,Dong, Xiaoyan,&Xie, Zhihong.(2020).Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(6),2715-2729. |
| MLA | Sun, Yu,et al."Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.6(2020):2715-2729. |
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