Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin | |
Wang, Xiaoyan; Yu, Shuangmei; Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen ; Li, Bowei; Li, Jinhua; Chen, Lingxin
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| 发表期刊 | TALANTA
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| ISSN | 0039-9140 |
| 2020-05-01 | |
| 卷号 | 211页码:120727 |
| 关键词 | RATIOMETRIC FLUORESCENCE OPTICAL-DETECTION GLYCOPROTEIN AFFINITY RECOGNITION SEPARATION STRATEGY SENSOR PH |
| 研究领域 | Chemistry, Analytical |
| DOI | 10.1016/j.talanta.2020.120727 |
| 产权排序 | [Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen; Li, Bowei; Li, Jinhua; Chen, Lingxin] Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Yu, Shuangmei] Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China |
| 通讯作者 | Wang, Xiaoyan([email protected]) ; Chen, Lingxin([email protected]) |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | We reported a facile strategy to assemble a ratiometric nanosensor for the ovalbumin (OVA) fluorescence determination and meanwhile it can be utilized for selective visual identification by naked eyes with fluorescent test papers under 365 nm UV lamp. The nanosensor was prepared through simply mixing blue color carbon dots (CDs) and green color core-shell imprinted polymers. Blue CDs were used directly as the internal reference without participating in the imprinting process and modified molecularly imprinted polymers (MIPs) were synthesized by post-imprinting, using fluorescein isothiocyanate (FITC) as fluorescence enhanced signal. Upon the addition of different concentrations of OVA, the fluorescence intensity of FITC was enhanced, while the fluorescence intensity of CDs was almost unchanged, leading to a detection limit as low as 15.4 nM. Accordingly, the fluorescence color was gradually changed from blue to dark olive green to green with naked eyes observation. Moreover, the ratiometric nanosensor was successfully applied to detect OVA in the human urine samples with satisfactory recoveries attaining of 92.0-104.0% with relative standard deviation (RSD) of 3.3-3.9% and 93.3-101.0% with RSDs of 2.7-3.8% for the spiked chicken egg white samples. This strategy reported here opens a novel pathway for biomacromolecule detection in real applications and can realize the visual observation on fluorescent test papers. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21804010, 21575159, 41776110, 21876199, 21976209] ; Science and Technology Development Plan of Shandong Province of China [2014GGF01074] ; Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050] ; Research Initiation Fund of Binzhou Medical University [BY2019KYQD39] ; Taishan Scholar Project Special Funding [ts20190962] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RATIOMETRIC FLUORESCENCE ; OPTICAL-DETECTION ; GLYCOPROTEIN ; AFFINITY ; RECOGNITION ; SEPARATION ; STRATEGY ; SENSOR ; PH |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000518708300036 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/25039 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 作者单位 | 1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 2.Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaoyan,Yu, Shuangmei,Wang, Jiangru,et al. Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin[J]. TALANTA,2020,211:120727. |
| APA | Wang, Xiaoyan.,Yu, Shuangmei.,Wang, Jiangru.,Yu, Jialuo.,Arabi, Maryam.,...&Chen, Lingxin.(2020).Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin.TALANTA,211,120727. |
| MLA | Wang, Xiaoyan,et al."Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin".TALANTA 211(2020):120727. |
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