| Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress | |
Huang, Y; He, N; Wang, YD; Zhang, LW ; Kang, Q; Wang, YQ; Shen, DZ; Choo, J; Chen, LX
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| 发表期刊 | JOURNAL OF MATERIALS CHEMISTRY B
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| ISSN | 2050-750X |
| 2019-04-21 | |
| 卷号 | 7期号:15页码:2557-2564 |
| 关键词 | OXIDATIVE STRESS IMAGING BASAL LIVE-CELL TURN-ON MITOCHONDRIA HCLO ANTIOXIDANTS ZEBRAFISH LYSOSOME |
| 研究领域 | Materials Science, Biomaterials |
| DOI | 10.1039/c9tb00079h |
| 产权排序 | [Huang, Yan; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Shandong, Peoples R China; [Huang, Yan; He, Na; Wang, Yude; Zhang, Liangwei; Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [He, Na; Wang, Yude] Binzhou Med Univ, Med Res Ctr, Inst Mol Med, Yantai 264003, Peoples R China; [Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 通讯作者 | Kang, Qi([email protected]) ; Choo, Jaebum([email protected]) ; Chen, Lingxin([email protected]) |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | Hypoxic stress is a common concern in medicine and biology, which can induce the cellular injury and death by excess production of reactive oxygen species (ROS). Hypochlorous acid (HOCl), one of the ROS, plays a crucial role in the oxidative damage to tissue proteins in the pathogenesis of various diseases. Therefore, excess production of HOCl might be an important factor for the damage caused by hypoxic stress. However, most of the present methods cannot meet the demand of real-time detection on account of the labile and reactive chemical properties of HOCl. Herein, we designed a near-infrared fluorescent probe, Cy-HOCl, for the selective imaging of HOCl in cells and in vivo. Cy-HOCl includes two moieties: a 4-amino-3-nitrophenol group as the response unit and a near-infrared heptamethine cyanine fluorophore as the fluorescent modulator. Cy-HOCl exhibits excellent selectivity and sensitivity towards the detection of HOCl. The hypoxic response behavior of Cy-HOCl is evaluated in cells to clarify the relationship between HOCl and hypoxia. The probe is also applied to measure HOCl in ex vivo-dissected organs of an acute ischemia mouse model as well as for real-time monitoring the changes of HOCl in the hypoxic zebrafish model. |
| 文章类型 | Article |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 81573393, 21575080] ; program of Youth Innovation Promotion Association, CAS [2017256, 2019217] ; National Research Foundation of KoreaNational Research Foundation of Korea [2017M3D1A1039287, 2018M3A7B4071203] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OXIDATIVE STRESS ; IMAGING BASAL ; LIVE-CELL ; TURN-ON ; MITOCHONDRIA ; HCLO ; ANTIOXIDANTS ; ZEBRAFISH ; LYSOSOME |
| 研究领域[WOS] | Materials Science, Biomaterials |
| WOS记录号 | WOS:000465281100014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24955 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Shandong, Peoples R China; 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Binzhou Med Univ, Med Res Ctr, Inst Mol Med, Yantai 264003, Peoples R China; 4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; 5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Y,He, N,Wang, YD,et al. Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(15):2557-2564. |
| APA | Huang, Y.,He, N.,Wang, YD.,Zhang, LW.,Kang, Q.,...&Chen, LX.(2019).Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress.JOURNAL OF MATERIALS CHEMISTRY B,7(15),2557-2564. |
| MLA | Huang, Y,et al."Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress".JOURNAL OF MATERIALS CHEMISTRY B 7.15(2019):2557-2564. |
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