| A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum | |
Yang, DL ; Han, YJ; Liu, YL; Cao, RW; Wang, Q; Dong, ZJ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2019-05 | |
| 卷号 | 88页码:441-448 |
| 关键词 | Ruditapes philippinarum Peptidoglycan recognition protein Immune recognition Immune response |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2019.03.017 |
| 产权排序 | [Yang, Dinglong; Han, Yijing; Liu, Yongliang; Cao, Ruiwen; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Han, Yijing; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Zhao, Jianmin([email protected]) |
| 作者部门 | 海岸带生物资源高效利用研究与发展中心 |
| 英文摘要 | Peptidoglycan recognition proteins (PGRPs) are important pattern recognition receptors in the innate immune system of invertebrates. In the study, a short PGRP (designed as RpPGRP) was identified and characterized from the manila clam Ruditapes philippinamm. The open reading frame of RpPGRP encoded a polypeptide of 249 amino acids with a calculated molecular mass of 27.2 kDa and an isoelectric point of 6.62. Multiple alignments and phylogenetic analysis strongly suggested that RpPGRP was a new member of the PGRP superfamily. In non stimulated clams, RpPGRP exhibited different tissue expression pattern, and highly expressed in hepatopancreas and hemocytes. Expression of RpPGRP transcripts was significantly up -regulated in hemocytes of clams post Vibrio crnguillartzn or Micrococcus luteus challenge. The recombinant RpPGRP (rRpPGRP) exhibited high affinity to PGN, LPS and zymosan in a concentration-dependent manner. With a broad spectrum of bacterial binding activities, rRpPGRP exhibited strong agglutination activity to Escherichia colt, Vibrio splendidus, V. anguiliarum and M. luteus. Furthermore, rRpPGRP exhibited Zn2+ -dependent amidase activity and catalyzed the degradation of insoluble PGN. Especially, rRpPGRP exhibited significant antibacterial activity against E. coil and M. luteus. Moreover, the biofilm formation of E. coil could be inhibited after rRpPGRP incubation in the presence of Zn2+. This inhibitory effect of rRpPGRP might attribute to its amide bactericidal activity. Taken together, rRpPGRP played important roles in PGRP-mediated immune defense mechanisms, especially by recognizing antigens and eliminating bacteria. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41476126, 41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KZZD-EW-14, KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Youth Innovation Promotion Association CAS [2016196] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | GRAM-NEGATIVE BACTERIA ; L-ALANINE AMIDASE ; MOLECULAR-CLONING ; BINDING PROTEIN ; RECEPTOR ; GENES ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; PURIFICATION ; EXPRESSION |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000465060500052 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24948 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室 支撑部门_中国科学院牟平海岸带环境综合试验站 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, DL,Han, YJ,Liu, YL,et al. A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:441-448. |
| APA | Yang, DL.,Han, YJ.,Liu, YL.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,88,441-448. |
| MLA | Yang, DL,et al."A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 88(2019):441-448. |
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| A peptidoglycan reco（2353KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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