| Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple | |
Jiang, SH; Wang, N; Chen, M ; Zhang, R; Sun, QG; Xu, HF; Zhang, ZY; Wang, YC; Sui, XQ; Wang, SF; Fang, HC; Zuo, WF; Su, MY; Zhang, J; Fei, ZJ; Chen, XS
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| 发表期刊 | PLANT BIOTECHNOLOGY JOURNAL
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| ISSN | 1467-7644 |
| 2020 | |
| 页码 | 13 |
| 关键词 | apple anthocyanin MdMYB1 promoter DNA methylation AGO4 RdDM |
| 研究领域 | Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1111/pbi.13337 |
| 产权排序 | [Jiang, Shenghui; Wang, Nan; Sun, Qingguo; Xu, Haifeng; Zhang, Zongying; Wang, Yicheng; Fang, Hongcheng; Zuo, Weifang; Su, Mengyu; Zhang, Jing; Chen, Xuesen] Shandong Agr Univ, Coll Hort Sci & Engn, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China; [Chen, Min] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zhang, Rui] Heze Univ, Heze, Peoples R China; [Sui, Xiuqi] Yantai Modern Fruit Ind Dev Co, Yantai Modern Fruit Ind Res Inst, Yantai, Peoples R China; [Wang, Sufang] Shaanxi Fruit Ind Ctr, Xian, Peoples R China; [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA |
| 通讯作者 | Chen, Xuesen([email protected]) |
| 作者部门 | 海岸带环境过程实验室 |
| 英文摘要 | Methylation at the MdMYB1 promoter in apple sports has been reported as a regulator of the anthocyanin pathway, but little is known about how the locus is recognized by the methylation machinery to regulate anthocyanin accumulation. In this study, we analysed three differently coloured 'Fuji' apples and found that differences in the transcript levels of MdMYB1, which encodes a key regulator of anthocyanin biosynthesis, control the anthocyanin content (and therefore colour) in fruit skin. The CHH methylation levels in the MR3 region (-1246 to -780) of the MdMYB1 promoter were found to be negatively correlated with MdMYB1 expression. Thus, they were ideal materials to study DNA methylation in apple sports. The protein of RNA-directed DNA methylation (RdDM) pathway responsible for CHH methylation, MdAGO4, was found to interact with the MdMYB1 promoter. MdAGO4s can interact with MdRDM1 and MdDRM2s to form an effector complex, fulfilling CHH methylation. When MdAGO4s and MdDRM2s were overexpressed in apple calli and Arabidopsis mutants, those proteins increase the CHH methylation of AGO4-binding sites. In electrophoretic mobility shift assays, MdAGO4s were found to specifically bind to sequence containing ATATCAGA. Knockdown of MdNRPE1 did not affect the binding of MdAGO4s to the c3 region of the MdMYB1 promoter in 35S::AGO4 calli. Taken together, our data show that the MdMYB1 locus is methylated through binding of MdAGO4s to the MdMYB1 promoter to regulate anthocyanin biosynthesis by the RdDM pathway. |
| 文章类型 | Article; Early Access |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31730080, 31701892] ; Ministry of Science and Technology Key Research and Development Plan [SQ2016YFSF030011] ; Agricultural Variety Improvement Project of Shandong Province [2019LZGC007] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MYB TRANSCRIPTION FACTOR ; DIRECTED DNA METHYLATION ; RED COLORATION ; NONCODING RNA ; SKIN COLOR ; FRUIT ; GENE ; EXPRESSION ; ACCUMULATION ; ARGONAUTE4 |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS记录号 | WOS:000510084500001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24763 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Shandong Agr Univ, Coll Hort Sci & Engn, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 3.Heze Univ, Heze, Peoples R China; 4.Yantai Modern Fruit Ind Dev Co, Yantai Modern Fruit Ind Res Inst, Yantai, Peoples R China; 5.Shaanxi Fruit Ind Ctr, Xian, Peoples R China; 6.Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA |
| 推荐引用方式 GB/T 7714 | Jiang, SH,Wang, N,Chen, M,et al. Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple[J]. PLANT BIOTECHNOLOGY JOURNAL,2020:13. |
| APA | Jiang, SH.,Wang, N.,Chen, M.,Zhang, R.,Sun, QG.,...&Chen, XS.(2020).Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple.PLANT BIOTECHNOLOGY JOURNAL,13. |
| MLA | Jiang, SH,et al."Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple".PLANT BIOTECHNOLOGY JOURNAL (2020):13. |
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| Methylation of MdMYB（4508KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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