| VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC | |
Zhou, Ling; Yi, Yuetao ; Yuan, Qing; Zhang, Jing; Li, Youjie; Wang, Pingyu; Xu, Maolei; Xie, Shuyang
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| 发表期刊 | FREE RADICAL BIOLOGY AND MEDICINE
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| ISSN | 0891-5849 |
| 2018-12 | |
| 卷号 | 129页码:177-185 |
| 关键词 | Vanadyl alginate oligosaccharides Non-small cell lung cancer ROS AKT PTEN |
| 研究领域 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| DOI | 10.1016/j.freeradbiomed.2018.09.016 |
| 产权排序 | [Zhou, Ling; Yuan, Qing; Zhang, Jing; Li, Youjie; Wang, Pingyu; Xie, Shuyang] Binzhou Med Univ, Dept Biochem & Mol Biol, Key Lab Tumor Mol Biol, Yantai 264003, Shandong, Peoples R China; [Zhou, Ling; Xu, Maolei] Binzhou Med Univ, Sch Pharm, Key Lab Tradit Chinese Med Prescript Effect & Cli, State Adm Tradit Chinese Med, Yantai 264003, Shandong, Peoples R China; [Yi, Yuetao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Previous studies have confirmed that protein tyrosine phosphatase 1B (PTP1B) can promote tumour progression in non-small cell lung cancer (NSCLC). Vanadyl alginate oligosaccharides (VAOS) is a new coordination compounds that possesses a good PTP1B inhibitory activity. However, the potent anticancer efficacy of VAOS in human NSCLC requires further study. In this study, VAOS exhibited effective inhibitory effects in NSCLC both in cultured cells and in a xenograft mouse model. VAOS was further identified to induce NSCLC cell apoptosis through activating protein kinase B (AKT) to elevate intracellular reactive oxygen species (ROS) levels by increasing in oxygen consumption and impairing the ROS-scavenging system. Neither silencing of PTP1B by siRNA nor transient overexpression of PTP1B had an effect on the AKT phosphorylation triggered by VAOS, indicating that PTP1B inhibition was not involved in VAOS-induced apoptosis. Through phosphorus colorimetric assay, we demonstrated that VAOS notably inhibited phosphatase and tensin homologue deleted on chromosome 10 (PTEN) dephosphorylation activity, another member of the protein tyrosine phosphatases (PTPases)-upstream factor of AKT. Interestingly, PTEN knockdown sensitized cells to VAOS, whereas ectopic expression of PTEN markedly rescued VAOS-mediated lethality. In vivo, VAOS treatment markedly reduced PTEN activity and tumour cell burden with low systemic toxicity. Thus, our data not only provided a new therapeutic drug candidate for NSCLC, but presented new understanding into the pharmacological research of VAOS. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [81772281] ; Natural Science Foundation of Shandong Province [ZR2018BH046, ZR2018QH005] ; Project of Shandong Province Higher Educational Science and Technology Program [J15LM51] ; Taishan Scholar Project of Shandong Province [ts201712067] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | HEPATOCELLULAR-CARCINOMA ; OXIDATIVE STRESS ; PTEN ; CANCER ; CELLS ; MODULATION ; MECHANISMS ; SENESCENCE |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| WOS记录号 | WOS:000450298400016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24590 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Binzhou Med Univ, Dept Biochem & Mol Biol, Key Lab Tumor Mol Biol, Yantai 264003, Shandong, Peoples R China; 2.Binzhou Med Univ, Sch Pharm, Key Lab Tradit Chinese Med Prescript Effect & Cli, State Adm Tradit Chinese Med, Yantai 264003, Shandong, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ling,Yi, Yuetao,Yuan, Qing,et al. VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC[J]. FREE RADICAL BIOLOGY AND MEDICINE,2018,129:177-185. |
| APA | Zhou, Ling.,Yi, Yuetao.,Yuan, Qing.,Zhang, Jing.,Li, Youjie.,...&Xie, Shuyang.(2018).VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC.FREE RADICAL BIOLOGY AND MEDICINE,129,177-185. |
| MLA | Zhou, Ling,et al."VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC".FREE RADICAL BIOLOGY AND MEDICINE 129(2018):177-185. |
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| VAOS, a novel vanady（2723KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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