| Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli | |
Ge, Baosheng; Chen, Yao; Yu, Qian; Lin, Xiaojun; Li, Jiqiang; Qin, Song
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| 发表期刊 | PROCESS BIOCHEMISTRY
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| ISSN | 1359-5113 |
| 2018-08 | |
| 卷号 | 71页码:23-30 |
| 关键词 | Phycocyanobilin Heme biosynthetic pathway 5-Aminolevulinic acid Regulation Combinatorial biosynthesis Escherichia coli |
| 研究领域 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| DOI | 10.1016/j.procbio.2018.05.011 |
| 产权排序 | [Ge, Baosheng; Chen, Yao; Yu, Qian; Lin, Xiaojun; Li, Jiqiang] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Due to its biochemical and spectroscopic properties, phycocyanobilin (PCB) is of special interest for a variety of applications, but the production of PCB from Spirulina is complicated and costly. Here we report the assembly and regulation of the entire heme biosynthetic pathway for combinatorial biosynthesis of PCB in Escherichia coli. Our results shows that a plasmid containing bicistronic expression of hoxi and pcyA yields 6.64 mg/L PCB, which is higher than that of plasmid with one promoter for both genes (p < 0.01). Up-regulation of hemB, hemG, hemH, hemA(s), and hemL individually or simultaneously does not significantly improve the yield of PCB, but induces accumulation of 5-Aminolevulinic acid (ALA) and heme. Increasing the copy number of hoxi and pcyA genes slightly improves the production of PCB by enhancing the expression level of Hox1 and PcyA enzymes, suggesting that this two-step enzymatic reaction is the rate-limiting step for the production of PCB. This work provides new important insights into the regulatory mechanism of the heme biosynthesis pathway for production of valuable chemicals. |
| 文章类型 | Article |
| 资助机构 | National key Research and Development Plan of China [2016YFE0106700] ; National Natural Science Foundation of China [21373271, 21673294] ; Key Research Project of Shandong Province [2017GGX40114] ; Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China [OF2015N009] ; Fundamental Research Funds for the Central Universities |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | 5-AMINOLEVULINIC ACID ; BILE-PIGMENTS ; ALLOPHYCOCYANIN ; TETRAPYRROLES ; CHROMOPHORE ; DISORDERS ; SPIRULINA ; EFFICIENT ; GLUCOSE ; PROTEIN |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| WOS记录号 | WOS:000442004200004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24506 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Baosheng,Chen, Yao,Yu, Qian,et al. Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli[J]. PROCESS BIOCHEMISTRY,2018,71:23-30. |
| APA | Ge, Baosheng,Chen, Yao,Yu, Qian,Lin, Xiaojun,Li, Jiqiang,&Qin, Song.(2018).Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli.PROCESS BIOCHEMISTRY,71,23-30. |
| MLA | Ge, Baosheng,et al."Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli".PROCESS BIOCHEMISTRY 71(2018):23-30. |
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| Regulation of the he（1488KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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