A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action

doi:10.1016/j.fsi.2018.06.019

	A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action
	Yang, Dinglong1,2 ; Zhang, Qianqian1,2 ; Wang, Qing1,2 ; Chen, Lizhu 3; Liu, Yongliang 1; Cong, Ming4 ; Wu, Huifeng4 ; Li, Fei4 ; Ji, Chenglong4 ; Zhao, Jianmin1,2
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2018-09-01
卷号	80 页码:274-280
关键词	Ruditapes philippinarum Defensin Antimicrobial activity Membrane disruption
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2018.06.019
产权排序	[Yang, Dinglong; Zhang, Qianqian; Wang, Qing; Liu, Yongliang; Zhao, Jianmin] Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China; [Yang, Dinglong; Zhang, Qianqian; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China; [Cong, Ming; Wu, Huifeng; Li, Fei; Ji, Chenglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
作者部门	中国科学院牟平海岸带环境综合试验站
英文摘要	Defensins are small cysteine-rich cationic proteins that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. In the present study, a defensin-like antimicrobial peptide (designed as RpdefB) was identified and characterized from the manila clam Ruditapes philippinarum. The open reading frame of RpdefB encoded a 70-amino acid polypeptide with a calculated molecular mass of 7.5 kDa and isoelectric point of 8.16. Multiple alignments and phylogenetic analysis strongly suggested that RpdefB was a new member of the defensin family. In non-stimulated clams, RpdefB transcripts were constitutively expressed in all five tested tissues, especially in the hepatopancreas. After Vibrio anguillarum challenge, expression of RpdefB mRNA in hemocytes was significantly up-regulated at 6h, 12 h and 72 h. The synthetic peptide RpdefB showed high antibacterial activity against the Gram-negative bacterium Vibrio splendidus. Moreover, membrane integrity analysis revealed that RpdefB increased the membrane permeability of Escherichia coli and then resulted in cell death. Overall, our results suggested that RpdefB played an important role in the elimination of invading bacterium, perhaps through membrane-disruptive activity.
文章类型	Article
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association CAS
收录类别	SCI
语种	英语
关键词[WOS]	ALLOMYRINA-DICHOTOMA DEFENSIN ; OYSTER CRASSOSTREA-GIGAS ; MOLECULAR CHARACTERIZATION ; ESCHERICHIA-COLI ; MARINE-INVERTEBRATES ; BIOFILM DEVELOPMENT ; ARTHROPOD DEFENSIN ; GENE-EXPRESSION ; INNATE IMMUNITY ; HOST-DEFENSE
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000440958100030
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24018
专题	中国科学院海岸带环境过程与生态修复重点实验室支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Cong, Ming; Zhao, Jianmin
作者单位	1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China 3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dinglong,Zhang, Qianqian,Wang, Qing,et al. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action[J]. FISH & SHELLFISH IMMUNOLOGY,2018,80:274-280.
APA	Yang, Dinglong.,Zhang, Qianqian.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2018).A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action.FISH & SHELLFISH IMMUNOLOGY,80,274-280.
MLA	Yang, Dinglong,et al."A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action".FISH & SHELLFISH IMMUNOLOGY 80(2018):274-280.