| Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments | |
Chen, Yulin1,2; Liu, Shiliang A.3; Mou, Haijin2; Ma, Yunxiao2; Li, Meng1; Hu, Xiaoke1
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| 发表期刊 | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 2017-05-16 | |
| 卷号 | 8页码:871 |
| 关键词 | Bacillus Licheniformis Purification Bacteriostat Structure Lc-esi-ms/ms Stabilization |
| 研究领域 | Microbiology |
| DOI | 10.3389/fmicb.2017.00871 |
| 产权排序 | [Chen, Yulin; Li, Meng; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Chen, Yulin; Mou, Haijin; Ma, Yunxiao] Ocean Univ China, Sch Food Sci & Engn, Qingdao, Peoples R China; [Liu, Shiliang A.] Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Antibiotic resistance has become one of the world's most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M + H](+) m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was R-n-Glu(1)-Leu/lle(2)-Leu(3)-Val(4)-Asp(5)-Leu(6)-Leu/Ile(7). The lipopeptides showed well resistance to UV light and the change of pH and temperature. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(41376138 ; North China Sea Branch of State Oceanic Administration(QDZC20150420-002) ; 41576165) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ANTIMICROBIAL PEPTIDES ; CYCLIC LIPOPEPTIDES ; VIBRIO-PARAHAEMOLYTICUS ; MS/MS CHARACTERIZATION ; MICROBIAL SURFACTANTS ; MASS-SPECTROMETRY ; IDENTIFICATION ; PURIFICATION ; SUBTILIS ; STRAINS |
| 研究领域[WOS] | Microbiology |
| WOS记录号 | WOS:000401575500003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/22514 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Ocean Univ China, Sch Food Sci & Engn, Qingdao, Peoples R China 3.Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA |
| 推荐引用方式 GB/T 7714 | Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,et al. Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments[J]. FRONTIERS IN MICROBIOLOGY,2017,8:871. |
| APA | Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,Ma, Yunxiao,Li, Meng,&Hu, Xiaoke.(2017).Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments.FRONTIERS IN MICROBIOLOGY,8,871. |
| MLA | Chen, Yulin,et al."Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments".FRONTIERS IN MICROBIOLOGY 8(2017):871. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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