The invention discloses a preparation method of food grade phycocyanin protein, comprising the following steps : breaking the wall of the spirulina power; adding weak acid salt buffer solution in the wall broken spirulina power for digestion; carrying out solid-liquid separation on the spirulina power leach liquor; extracting supernatant; filtering the supernatant to obtain the crude extract; and purifying the crude extract to obtain the concentrated solution; and spraying and drying the concentrated solution. The invention also discloses a food grade phycocyanin protein prepared by the method, wherein the purity (OD620/OD280) is larger than 1.0; the content of the phycocyanin protein is larger than 50%; the content of allophycocyanin is larger than 10%; the content of phycoerythrin is larger than 2%; and the content of other proteins is smaller than 2%. The method has low production cost, is simple and effective, and is suitable for preparing mass food grade phycocyanin proteins.
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