The invention belongs to the field of producing protein through microbiological fermentation, and in particular relates to a microbiological preparation method of inulin source recombinant allophycocyanin. The method comprises the following steps : with inulin as raw material, degrading the raw material as a seed solution, culturing Escherichia coli P2 through a shaking table or a fermentation tank under the effect of a fermentation medium, and then inductively expressing through isopropyl-beta-D-thiopyran galactoside (IPTG) to obtain the inulin source recombinant allophycocyanin. The recombinant allophycocyanin can be applied to the fields of food, healthcare and medical and functional materials. The preparation method of the inulin source recombinant allophycocyanin is simple, and an application path with low cost and high yield is found for the bio-utilization of the inulin; compared with a method of using glucose as a carbon source, the microbiological preparation method disclosed by the invention has the advantage that the raw material cost of producing the allophycocyanin in fermentation production is greatly reduced.
; 席海瑞; 张锐; 宋璐非; 王绍宇; 刘冰
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