Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods | |
Wang, Beibei1,2; Liu, Wuxing1; Liu, Xiaoyan1,2; Franks, Ashley E.3; Teng, Ying1; Luo, Yongming1,4 ; Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [email protected]
| |
| 发表期刊 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| ISSN | 0048-9697 |
| 2017-07-15 | |
| 卷号 | 590页码:297-303 |
| 关键词 | Ddt-degrading Microorganisms Microbial Community Culture-dependent Culture-independent Community Diversity |
| DOI | 10.1016/j.scitotenv.2017.03.004 |
| 产权排序 | [Wang, Beibei; Liu, Wuxing; Liu, Xiaoyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Beibei; Liu, Xiaoyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Franks, Ashley E.] La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Microcosms for enrichment of DDT degrading microorganisms were monitored using culture-dependent and -independent methods. Culture dependent methods isolated several strains with DDT degradation potential, Pseudomonas species being the most frequent. One isolate, Streptomyces sp. strain D3, had a degradation rate of 77% with 20 mg L-1 of DDT after 7 days incubation, D3 also had degradation rates of 75% and 30% for PCB77 (3,3',4,4'-tetrachloro biphenyl) and PCNB (pentachloronitrobenzene) respectively. Culture-independent high throughput sequencing identified a different subset of the microbial community within the enrichment microcosms to the culture dependent method. Pseudomonas, the most frequently isolated strain, only represented the 12th most abundant operational taxonomic unit in the sequencing dataset (relative abundance 0.9%). The most frequently observed bacterial genus in the culture-independent analysis did not correspond with those recovered by culture-dependent methods. These results suggested that deep sequencing followed by a targeted isolation approach might provide an advantageous route to bioremediation studies. (C) 2017 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Key Research Program of the Chinese Academy of Sciences(KFZD-SW-303) ; National Natural Science Foundation of China(41671325 ; U1662110) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ORGANOCHLORINE PESTICIDES ; SEA BACTERIOPLANKTON ; AGRICULTURAL SOILS ; CONTAMINATED SOIL ; DEGRADATION ; CHINA ; WATER ; BIODEGRADATION ; DIVERSITY ; PENTACHLORONITROBENZENE |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000399511800031 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/22047 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [email protected] |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia 4.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,et al. Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,590:297-303. |
| APA | Wang, Beibei.,Liu, Wuxing.,Liu, Xiaoyan.,Franks, Ashley E..,Teng, Ying.,...&Liu, WX .(2017).Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods.SCIENCE OF THE TOTAL ENVIRONMENT,590,297-303. |
| MLA | Wang, Beibei,et al."Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods".SCIENCE OF THE TOTAL ENVIRONMENT 590(2017):297-303. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Comparative analysis（665KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论