产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递

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	产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递
其他题名	Potential direct interspecies electron transfer (DIET) from Clostridium spp. to Methanosarcina barkeri in amethanogenic isolation
	李莹 ; 郑世玲; 张洪霞 ; 王炳臣 ; 王欧美 ; 刘芳华
发表期刊	微生物学通报
ISSN	0253-2654
	2016-05-24
页码	13
关键词	种间直接电子传递革兰氏阳性菌 Clostridium Spp. Geobacter Methanosarcina Barkeri
产权排序	中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室;中国科学院大学;滨州医学院;
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	【目的】革兰氏阴性菌Geobacter metallireducens可以与乙酸型产甲烷菌Methanosaeta harundinacea或Methanosarcina barkeri通过种间直接电子传递(DIET)还原CO2产甲烷。本实验室前期的研究发现Methanosarcina mazei和Geobacteraceae在铁还原富集培养中形成团聚体,可能存在直接电子传递。然而,革兰氏阳性菌(如Clostridium spp.)与产甲烷菌是否存在种间直接电子传递尚不明确。【方法】采用Hungate厌氧滚管法,以乙醇为唯一电子供体从铁还原富集培养体系中获得产甲烷分离物(S6)。通过T-RFL...
中文摘要	Geobacter metallireducens, a Gram-negative bacterium, can directly transfer electron to acetoclastic methanogens such as Methanosaeta harundinacea and Methanosarcina barkeri for reducing carbon dioxide to methane. Our previous results showed that Methanosarcina mazei and Geobacteraceae formed aggregates in an iron (III)-reducing enrichment culture indicating direct interspecies electron transfer. However, the capability of direct electron transfer with methanogens for Gram-positive iron (III)-reducing bacteria such as Clostridium spp. is still unknown. [Methods] In this further study, a methanogenic isolation (S6) was achieved from the iron (III)-reducing enrichment by roll-tube (Hungate) method with ethanol as the sole electron donor. We used terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis to investigate the community of S6 and used electrochemical method such as cyclic voltammetry (CV) to confirm the electroactivity of isolation. [Results] Clone library analysis of 16S rRNA gene showed that Clostridium spp. (close to C. tunisiense) and Methanosarcina barkeri was predominant in the bacterial and archaeal community, respectively. Interestingly, addition of G. metallireducens into S6 did not increase the ability of both iron (III) reduction and methanogenesis, indicating that Clostridium spp. may play a similar role in direct interspecies electron transfer from G. metallireducens to M. barkeri. Furthermore, current generation of the S6 suspension dramatically decreased when contact between the organisms and the electrodes was prevented by dialysis bag, and CV showed no obvious redox peaks. [Conclusion] These results suggested that there was direct electron transfer in the methanogenic isolation. This work demonstrate that the dominant Gram-positive Clostridium spp. can potentially directly transfer electron to M. barkeri in a methanogenic isolation.
文章类型	期刊论文
资助机构	国家自然科学基金项目(No. 41371257，41573071，41401285)，中国科学院重点资助项目(KZZD-EW-14) ; 中国科学院百人计划经费资助(A 类)
收录类别	CSCD
语种	中文
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17267
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	王欧美
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室 2.中国科学院大学 3.滨州医学院
推荐引用方式 GB/T 7714	李莹,郑世玲,张洪霞,等. 产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递[J]. 微生物学通报,2016:13.
APA	李莹,郑世玲,张洪霞,王炳臣,王欧美,&刘芳华.(2016).产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递.微生物学通报,13.
MLA	李莹,et al."产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递".微生物学通报 (2016):13.

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