己烯雌酚及代谢物在鲫鱼体内的组织分布及药物代谢动力学

doi:10.16429/j.1009-7848.2015.11.027

YIC-IR > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源保护实验室

	己烯雌酚及代谢物在鲫鱼体内的组织分布及药物代谢动力学
其他题名	Tissue Distribution and Pharmacokinetics of Diethylstilbestrol and Its Metabolite Dienestrol in Carassius auratus
	田秀慧 ; 薛敬林 ; 邓旭修 ; 徐英江 ; 宫向红 ; 夏传海
发表期刊	中国食品学报
ISSN	1009-7848
	2015-11-30
卷号	15 期号:11 页码:178-185
关键词	己烯雌酚双烯雌酚鲫鱼药物代谢动力学超高效液相色谱-串联质谱法
DOI	10.16429/j.1009-7848.2015.11.027
产权排序	中国科学院烟台海岸带研究所;山东省海洋资源与环境研究院;中国科学院大学;
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	为全面了解己烯雌酚在鲫鱼体内的残留、消除和药物动力学特征,采用超高效液相色谱-串联质谱法,研究口服灌药给药时己烯雌酚及其主要代谢物双烯雌酚在鲫鱼体内的组织分布和药物代谢动力学。在水温(12±2)℃条件下,鲫鱼口服灌药0.1,1.0 mg/(kg bw)和10.0 mg/(kg bw)后,其血浆、肌肉和肝脏中己烯雌酚浓度-时间曲线关系符合一级吸收的二室开放动力学模型。以剂量1.0 mg/(kg bw)为例,3种组织中己烯雌酚峰值水平肝脏最高,肌肉次之,血浆最低,己烯雌酚的平均消除速率均较快,分别为26.7μg/(L·h),3.75μg/(kg·h)和9.26μg/(kg·h),在12,144 h...
中文摘要	Tissue distribution and pharmacokinetics of Diethylstilbestrol and its metabolite Dienestrol in plasma,muscle and liver of Carassius auratus under laboratory conditions using single oral administration was studied by ultra performance liquid chromatogram tandem mass spectrometry in this work. After administration by single oral of 0.1, 1.0mg/(kg bw) and 10.0 mg/(kg bw), Diethylstilbestrol concentrations in plasma, muscle and liver versus time were well described by a two-department open model with first-order absorption. For example, after single oral of 1.0 mg/(kg bw),the biggest concentration of Diethylstilbestrol in liver was highest, the next was muscle and the lowest was plasma. The average elimination speed was 26.7 μg/(L·h), 3.75 μg/(kg·h) and 9.26 μg/(kg·h) of plasma, muscle and liver, showing that Diethylstilbestrol could eliminate fast in Carassius auratus. Diethylstilbestrol was no detected after oral administration of 12 h, 144 h and 144 h. The variation of Dienestrol concentrations in plasma, muscle and liver of Carassius auratus was similar with Diethylstilbestrol, first increased and then decreased. Concentrations of Dienestrol in plasma, muscle and liver could reach peak after oral administration of 3, 10 h and 8 h, and no detected after 12, 144 h and 144 h. DAS2.0 pharmacokinetic parameter calculation program was used to process Diethylstilbestrol content-time datas and to calculate the relevant pharmacokinetic parameters of Diethylstilbestrol. For example, in the concentration of 1.0 mg/(kg bw),the results indicated that area under concentration-time curve(AUC) differed greatly in plasma, muscle and liver of Carassius auratus and the tissues had different accumulation capability. The overall clearance rate of Diethylstilbestrol in plasma, muscle and liver was 1.041, 0.121 L/(h·kg) and 0.052 L/(h·kg), indicating distribution was dispersed and elimination was fast. Under the experimental conditions, when Carassius auratus using single oral administration of0.1, 1.0 mg/(kg bw) and 10.0 mg/(kg bw), it was recommended withdrawal period of 4, 6 d and 7 d respectively. When the water temperature was highter than the experimental conditions, the withdrawal period could be shorten; and extended in lower water temperature. The concentrations versus time curves of Diethylstilbestrol in Carassius auratus provided useful information for studying decontamination of Carassius auratus that had Diethylstilbestrol contamination.
文章类型	期刊论文
资助机构	烟台市科技发展计划项目(2012134)
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:5586878
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17261
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.中国科学院烟台海岸带研究所 2.山东省海洋资源与环境研究院 3.中国科学院大学
推荐引用方式 GB/T 7714	田秀慧,薛敬林,邓旭修,等. 己烯雌酚及代谢物在鲫鱼体内的组织分布及药物代谢动力学[J]. 中国食品学报,2015,15(11):178-185.
APA	田秀慧,薛敬林,邓旭修,徐英江,宫向红,&夏传海.(2015).己烯雌酚及代谢物在鲫鱼体内的组织分布及药物代谢动力学.中国食品学报,15(11),178-185.
MLA	田秀慧,et al."己烯雌酚及代谢物在鲫鱼体内的组织分布及药物代谢动力学".中国食品学报 15.11(2015):178-185.

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