Whole genome analysis of halotolerant and alkalotolerant plant growth-promoting rhizobacterium Klebsiella sp D5A

doi:10.1038/srep26710

	Whole genome analysis of halotolerant and alkalotolerant plant growth-promoting rhizobacterium Klebsiella sp D5A
	Liu, WX ; Wang, QL ; Hou, JY ; Tu, C; Luo, YM; Christie, P ; Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [email protected]
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
	2016-05-24
卷号	6
DOI	10.1038/srep26710
产权排序	[Liu, Wuxing; Wang, Qingling; Hou, Jinyu; Christie, Peter] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Tu, Chen; Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	This research undertook the systematic analysis of the Klebsiella sp. D5A genome and identification of genes that contribute to plant growth-promoting (PGP) traits, especially genes related to salt tolerance and wide pH adaptability. The genome sequence of isolate D5A was obtained using an Illumina HiSeq 2000 sequencing system with average coverages of 174.7x and 200.1x using the paired-end and matepair sequencing, respectively. Predicted and annotated gene sequences were analyzed for similarity with the Kyoto Encyclopedia of Genes and Genomes (KEGG) enzyme database followed by assignment of each gene into the KEGG pathway charts. The results show that the Klebsiella sp. D5A genome has a total of 5,540,009 bp with 57.15% G + C content. PGP conferring genes such as indole-3-acetic acid (IAA) biosynthesis, phosphate solubilization, siderophore production, acetoin and 2,3-butanediol synthesis, and N2 fixation were determined. Moreover, genes putatively responsible for resistance to high salinity including glycine-betaine synthesis, trehalose synthesis and a number of osmoregulation receptors and transport systems were also observed in the D5A genome together with numerous genes that contribute to pH homeostasis. These genes reveal the genetic adaptation of D5A to versatile environmental conditions and the effectiveness of the isolate to serve as a plant growth stimulator.
文章类型	Article
资助机构	Key Research Program of the Chinese Academy of Sciences(KFZD-SW-303) ; National Natural Science Foundation of China(41001182 ; Key Projects in the National Science and Technology Pillar Program(2015BAD05B04) ; 41201313)
收录类别	SCI
语种	英语
关键词[WOS]	PSEUDOMONAS-CHLORORAPHIS O6 ; NITROGEN-FIXATION ; ESCHERICHIA-COLI ; PYRROLOQUINOLINE QUINONE ; PHOSPHATE SOLUBILIZATION ; 2-COMPONENT SYSTEM ; STUTZERI A1501 ; PH ; BACTERIA ; RESISTANCE
研究领域[WOS]	Science & Technology - Other Topics
WOS记录号	WOS:000376344500002
引用统计	被引频次：86[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17073
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [email protected]
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat 2.Chinese Acad Sci, Inst Coastal Zone Res
推荐引用方式 GB/T 7714	Liu, WX,Wang, QL,Hou, JY,et al. Whole genome analysis of halotolerant and alkalotolerant plant growth-promoting rhizobacterium Klebsiella sp D5A[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, WX.,Wang, QL.,Hou, JY.,Tu, C.,Luo, YM.,...&Liu, WX .(2016).Whole genome analysis of halotolerant and alkalotolerant plant growth-promoting rhizobacterium Klebsiella sp D5A.SCIENTIFIC REPORTS,6.
MLA	Liu, WX,et al."Whole genome analysis of halotolerant and alkalotolerant plant growth-promoting rhizobacterium Klebsiella sp D5A".SCIENTIFIC REPORTS 6(2016).

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